Miniature patch clamp amplifier
Sophion Bioscience develops custom made patch clamp
amplifiers. They feature automatic compensation for series
resistance (Rs) and cell capacitance (Cslow), and up to 300 nA
maximal current measured, depending on amplifier type.
From the platform of the screening station, a "bed-of-nails" connects
the patch clamp electrodes with the amplifier front ends. This
distance is kept minimal to reduce any noise pick-up from the
Several parallel patch clamp amplifiers are implemented on a
standard printed circuit board, enabling all amplifiers to be
positioned near the patch clamp electrodes. Due to their small size
these amplifiers are ideally suited for extensive parallelism and
provide considerable scalability.
100 % Rseries compensation
The QPatch amplifiers provide a unique series resistance
(Rseries) compensation feature that contributes to high quality
recordings. This feature allows for 100% Rs compensation.
Typically, patch clamp amplifiers are capable of compensating 50 -
95% of Rs, but oscillations occur at the higher compensation
percentages. The unique technology employed by the QPatch
amplifiers provides 100% Rs compensation without oscillations. This
feature prevents the possibility of false positives due to Rseries
The latest improvements (see
illustration) allow full Rs compensation at a speed that
accommodates even the fastest sodium ion channel. See the technical
Sophion Bioscience has an exclusive license to this technology
from Alembic Instruments. For more
information about this technology, please refer to the Alembic
Instruments website (http://www.alembicinstruments.com), or the
Resistance Compensation for Whole-Cell Patch-Clamp Studies Using a
Membrane State Estimator.